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After two consecutive weeks of unilateral lower extremity immobilization, walking recovery, and resistance exercise interventions in healthy middle-aged men, a significant downregulation of PGC-1, mitochondrial fission process 1 (MTFP1), TFAM, and DRP1 mRNA levels was found, along with a slight decrease in NRF1 and MFN1 protein levels in the skeletal muscle tissue of middle-aged men during the period of unilateral lower extremity immobilization

Further, blood-gas analysis on admission also revealed metabolic acidosis (pH, 7.340