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Nevertheless involvement in the audit stimulate a considerable change in behaviour among participating doctors, leading to subsequent improvement in their use of vitamin B12

injection of 50 mg/kg sodium pentobarbital (Sigma), and thoracic aorta was carefully dissected and maintained at 37 C in Krebs solution containing 118.2 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO 4 , 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.9 mM CaCl 2 and 11.7 mM dextrose (pH 7.4, 95% O 2 , 5% CO 2 )

In explaining its decision, the agency flagged concerns about complexities with regard to peptide-related impurities and active pharmaceutical ingredient (API) characterization, the potential for aggregation and a lack of human safety data