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Cell viability was calculated as a ratio of the absorbance in the treated group to that in the control group, which consisted of cells cultured under normal conditions without OGD/R exposure

IF and IHC staining of tissue sections Tumour tissue sections were deparaffinized in Histo-Clear (Scientific Laboratory Supplies) for 5 min, rehydrated through a series of decreasing ethanol concentrations, followed by a final 5 min wash in distilled water (dH 2 O)

Some feel better and others see no change

IL-27 siRNA can reverse this phenomenon, indicating that glutathione redox regulation of T-cell polarization depends on IL-27 production (69)