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Off-label use may lead to unintended side effects or ineffective treatment, as the medication may not be suitable for the intended condition

The relationship between tirzepatide and menstrual irregularities is primarily indirect, mediated through significant weight loss and metabolic improvements

A systematic review and meta-analysis published in eClinicalMedicine reported pooled mean weight regain of 5.63 kg and 5.81 percent of body weight

Following dissociation, islets cells were washed 3 times with PBS containing 0.01% BSA, followed by centrifugation at 352 g for 5 minutes, and then resuspended in PBS containing 0.01% BSA at a concentration of approximately 250,000 cells/mL, which was used for downstream DROP-Seq and DART-Seq experiments