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3D refinement was performed, using a low pass filtered RELION consensus map as an initial model and a generous mask created in cryoSPARC as default

The major difference is that whereas only the main chain carboxylate of wild-type Thr1273 interacts with basic Lys17 on the WD40 surface, both the side chain and main chain carboxylate groups of the clientized Glu1273 residue form extensive interactions with a WD40 basic cluster of Arg15, Lys17, and Arg272 (Fig

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Her practice focuses on womens, reproductive, and sexual health, with particular expertise in conditions such as endometriosis, PCOS, vulvar disorders, chronic vaginal infections, and hormone-related concerns