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Slides containing retinal sections were deparaffinized by immersing the slides in various subsequent ethanol dilutions(100%, 95%,70% and 50%), then rinsed with phosphate-buffered saline, pH 7.4 (PBS, Quality Biological, 119-069-101), followed by incubation in blocking buffer containing 0.5% normal donkey serum (Abcam, ab7475), 0.5% bovine serum albumin (BSA, GoldBio, A-420-100), 0.1% Triton X-100 (Sigma, T8787-100) in PBS pH 7.4 for 2 h at room temperature 28

BMC Public Health 15 , 1208 (2015)

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1 6 The second is ex vivo human hair-follicle organ culture , in which isolated follicles are kept alive in a dish and their elongation measured over days