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To further assess the contribution of melatonin on the circadian clocks, BMAL1 was knocked down with siRNA in Hep3B cells that were treated with or without melatonin (0.5 or 1 mM) for 24 hours after transfection

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In both groups, 46.37% had type 2 diabetes and 38.1% had elevated fasting blood glucose levels, 29% reported nicotine dependence and 4.9% had alcohol-related disorders