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For a cardiovascular performance assay, six endpoints were chosen to evaluate the cardiovascular performance in the zebrafish larvae: the heart rate and time interval in ventricles, stroke volume, cardiac output, ejection fraction, and shortening fraction

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During the treatment process, nanomedicine (5 mg/kg) was injected into the tail vein of mice on days 0 and 5, respectively, and US irradiation (10 min, 1.5 W/cm 2 , 1 MHz, 50% duty cycle) was performed on days 1 and 6