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Sodium phosphate (50 mM pH 7.4), EDTA (1 mM), GSH (2 mM), NADPH (0.25 mM), glutathione reductase (1 U) and samples (2.5 M) were mixed in a cuvette at 37C

B-FAHF-2 demonstrated protection from anaphylaxis in a PNA mouse model, with partial protection persisting for up to 50 weeks [30]

The addition of the N-terminal tyrosine residue was incorporated to facilitate radiolabelling for pharmacokinetic research studies, and has since become a standard part of the compounds structure as used in laboratory research