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First, samples were processed by adding lysis buffer (1.5% SDS/100 mM Tris-Cl), homogenized, and centrifuged to obtain supernatants

When less food is eaten or when digestion slows, the body may: Break down more fat or protein for energy Produce more ammonia-like waste products Release compounds that are later filtered out through the kidneys These changes do not happen in everyone, but they may contribute to urine odor differences for some people

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Chronic exposure to high-sugar foods can alter these reward circuits, potentially leading to heightened cravings and a dependence on sugar