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Pull-down assay For testing the Sh HTL7 interaction with Sh MAX2 At ASK1 and At SMAX1 (full length, D2 domain and corresponding mutants) or At SMXL6/ At SMXL7 using GST- Sh HTL7 as bait, reaction mixtures of 100 L in 20 mM HEPES pH 7.5, 200 mM NaCl, 0.5 mM TCEP buffer were prepared for a final concentration of roughly 3.5 M GST- Sh HTL7 and 5 M for each of the tested binding partners