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Reducing oxidative stress is important for hair because high free radical activity upregulates the enzyme that converts testosterone to DHT locally, amplifying follicle damage beyond what circulating DHT alone would cause

Added benzyl alcohol does the work here as a preservative, as it allows for multiple withdrawals from the same vial, making it a reusable and reliable option in many clinical and home-care settings

Briefly, aliquots of 250 L were taken of each urine culture and diluted with pure methanol (1:3 for clinical samples and 1:6 for the in vitro analysis), vortexed, incubated on ice for 30 min, and centrifuged at x16000 g for 10 min