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After fixation, the cells were washed again with PBS and incubated overnight at 4 C with primary antibodies in incubation buffer (0.1% saponin, 0.1% Tween-20, and 5% FBS in PBS)

Benefits of fluoroscopy-guided injections include: Confirmed accuracy: Direct visualization ensures medication enters the joint space Reduced complications: Minimizes risk of soft tissue injection and associated inflammation Improved outcomes: Patients experience better pain relief when medication reaches the intended target [7] Enhanced safety: Real-time imaging helps avoid blood vessels, nerves, and other structures Studies demonstrate that fluoroscopy-guided injections result in significant pain relief and improved joint function compared to those who received traditional blind injections [7]