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Five micrograms of the peptide were loaded onto a C18-reversed-phase column in buffer A (2% acetonitrile and 0.1% formic acid) and separated with a linear gradient of buffer B (80% acetonitrile and 0.1% formic acid) at a flow rate of 250 nL min 1 controlled by IntelliFlow technology over 120 min
Disappointingly, once stopped, patients notice a gradual regain of up to two-thirds of the weight they lost, Dr Yates told The Conversation
Hair follicles, stressed by rapid caloric restriction and weight change, shed at higher rates
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